JATROPHA GAUMERI PDF

Jatropha gaumeri Greenm.; pomol che’. Tree (or shrub) with milky latex; leaves alternate, simple, broadly ovate, base cordate, margin entire, apex acuminate to. Jatropha gaumeri is a species of plants with 18 observations. Family: Euphorbiaceae Juss. Genus: Jatropha L. Jatropha gaumeri Greenm. This species is accepted, and its native range is SE. Mexico (and Tamaulipas) to.

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Bioactive Terpenoids from Roots and Leaves of Jatropha gaumeri. Recibido el 2 de octubre del Aceptado el 20 de febrero del The methanolic extracts of roots and leaves of Jatropha jatopha showed antimicrobial and antioxidant activity, respectively. The bioassay-guided purification of the root extract resulted in the isolation and identification of 2- epi -jatrogrossidione 1a rhamnofolane diterpene with antimicrobial activity, and of epi -4 E- jatrogrossidentadione 2a lathyrane diterpene without biological activity.

We wish to report here the isolation of the metabolites mentioned above and the complete assignment, not reported to date, of the 13 C NMR signals for 1. Various medicinal properties have been attributed to plant species of the large genus Jatropha Euphorbiaceae. For instance, the fresh latex of many plants belonging to this genus is used in folk medicine for the treatment of mouth blisters [1], pimples [2], and scabies [3]; similarly, leaf infusions are used to treat ulcers [4], jztropha wounds [5], and diarrhea [6]; finally, both the leaves and seeds of some Jatropha spp.

Phytochemically, the genus Jatropha is recognized as an important source of numerous structural classes of secondary metabolites, including alkaloids [9], diterpenes [], lignans [13], triterpenes [14], and cyclic peptides [15].

A number of biological activities have been detected in natural products from Jatropha spp. One of the most frequently used plants in Yucatecan traditional medicine is Jatropha gaumeri Greenm.

The baumeri grows at sea level in the jungles of Guatemala and Belize, and in the states of Quintana Roo and Yucatan, in Mexico [19].

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When cut, the tree exudates a milky resin which is used to alleviate skin rashes and mouth blisters, as well as to treat fever and bone fractures [1, 20]. As part of an ongoing screening project directed towards the search for biologically active metabolites from the Yucatecan flora [21], we wish to describe herein the bioassay-directed purification of the root and leaf extracts of J. A bioassay-guided purification of the antimicrobial root organic crude extract of J.

A complete assignment of the 13 C NMR spectrum signals for the structure of 1not previously reported in the literature, is detailed in Experimental. Inactive epi -4 E -jatrogrossidentadione 2 was isolated during the purification of 1. This lathyrane-type diterpene was previously isolated from the roots of J.

The methanolic extract obtained from the leaves of J. The second, less-polar, fraction showed the presence of two main components having very similar R f values on TLC; all attempts to separate the components in their natural form proved unsuccessful.

Esterification and oxidation of the two-component mixture produced the corresponding acetylated and keto derivatives, which could then be separated by means of PTLC on AgNO 3 -impregnated silica gel plates [27, 28]. The second component, obtained in pure form, was identified as the triterpene alcohol taraxasterol by comparing its spectroscopic data with those reported in the literature [29,30].

Chromatographic purifications were run using silica gel 60 Merck, mesh for open column, silica gel 60 GF for VLC, and silica gel 60 Merck, mesh for flash column.

Roots and leaves of J.

The extracts were combined and the solvent eliminated under reduced pressure to yield Testing of both jatrooha methanolic crude extract 1A and the resulting crude fractions 2A and 2B for antimicrobial activity using the agar-overlay method [21], indicated that fraction 2A had the strongest activity against Bacillus subtilis ATCC A bioassay guided purification of fraction 2A Fraction 3H mg was further purified by flash CC 4.

The organic crude extract was suspended in a 3: The low polarity fraction was subjected to a VLC purification Esterification of fraction 8F. A mixture of 8F 33 mgacetic anhydride 1 mL and pyridine 0. The organic layer was successively washed 1: Hydrolisis of the acetylated derivatives 9A and 9B. Each fraction 9A, 8. The organic layer was washed with water and brine and dried over anhydrous sodium sulfate.

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Evaporation of the solvent yielded the corresponding hydrolyzed product in good yield, i. Oxidation of fraction 8F. The reaction mixture was passed through a silica gel bed mesh, 3 cm high and the adsorbent washed with CH 2 Cl 2 to produce 18 mg of crude oxidized product.

Antimicrobial assay by the agar overlay method. Bacillus subtilis ATCC jatropba used for testing antimicrobial activity.

Bioactive Terpenoids from Roots and Leaves of Jatropha gaumeri

Tripticasein soy agar Bioxon was used as solid medium. The TLC plates were placed in a sterile Petri dish and covered with 4. Biological activity was observed as clear inhibition zones against the purple background.

Amikacin was used as positive control. After drying, the plate was sprayed with a 0. Active products remained as orange spots on a white background. All experiments were carried out in duplicate. Active products appeared as yellow spots against a purple background. Colorless gum; R f 0.

Jatropha gaumeri Greenm.

Plants4 Fitoterapia66 Phytochemistry50 Phytochemistry23 Phytochemistry51 Phytomedicine8 Phytochemistry27 Jahropha31 Phytochemistry22 Methods in Plant BiochemistryVol. Springer-Verlag, 2 nd edition, New York, Techniques and ApplicationsVol. Marcel Dekker, USA, Phytochemistry20 Phytochemistry43 Abstract The methanolic extracts of roots and leaves of Jatropha gaumeri showed antimicrobial and antioxidant activity, respectively.

Introduction Various medicinal properties have been attributed to plant species of the large genus Jatropha Euphorbiaceae. Results and discussion A bioassay-guided purification of the antimicrobial root organic crude extract of J.

Yaumeri material Roots and leaves of J. Extraction gaumfri isolation The roots 2.